diff --git a/devtools/projects/ng-devtools-backend/src/lib/profiling/BUILD.bazel b/devtools/projects/ng-devtools-backend/src/lib/profiling/BUILD.bazel
index fb96f4c77f1..c72f4a6fdb1 100644
--- a/devtools/projects/ng-devtools-backend/src/lib/profiling/BUILD.bazel
+++ b/devtools/projects/ng-devtools-backend/src/lib/profiling/BUILD.bazel
@@ -1,4 +1,4 @@
-load("//devtools/tools:defaults.bzl", "ts_project")
+load("//devtools/tools:defaults.bzl", "ts_project", "ts_test_library", "zoneless_web_test_suite")
 
 package(default_visibility = ["//devtools:__subpackages__"])
 
@@ -21,3 +21,16 @@ ts_project(
         "//devtools/projects/protocol",
     ],
 )
+
+ts_test_library(
+    name = "performance_track_test_lib",
+    srcs = ["performance-track.spec.ts"],
+    deps = [
+        ":profiling",
+    ],
+)
+
+zoneless_web_test_suite(
+    name = "performance_track_test",
+    deps = [":performance_track_test_lib"],
+)
diff --git a/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.spec.ts b/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.spec.ts
new file mode 100644
index 00000000000..4a847045b41
--- /dev/null
+++ b/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.spec.ts
@@ -0,0 +1,31 @@
+/**
+ * @license
+ * Copyright Google LLC All Rights Reserved.
+ *
+ * Use of this source code is governed by an MIT-style license that can be
+ * found in the LICENSE file at https://angular.dev/license
+ */
+
+describe('performance track', () => {
+  const globalWithAngularDebugApi = window as unknown as {ng?: unknown};
+  let angularDebugApi: unknown;
+
+  beforeEach(() => {
+    angularDebugApi = globalWithAngularDebugApi.ng;
+    globalWithAngularDebugApi.ng = {};
+  });
+
+  afterEach(() => {
+    if (angularDebugApi === undefined) {
+      delete globalWithAngularDebugApi.ng;
+    } else {
+      globalWithAngularDebugApi.ng = angularDebugApi;
+    }
+  });
+
+  it('does not throw an error when initialized in development mode', async () => {
+    const performanceTrack = await import('./performance-track');
+
+    expect(() => performanceTrack.enablePerformanceTrack()).not.toThrow();
+  });
+});
diff --git a/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.ts b/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.ts
index da9653769cf..8c0dfa14085 100644
--- a/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.ts
+++ b/devtools/projects/ng-devtools-backend/src/lib/profiling/performance-track.ts
@@ -16,9 +16,6 @@ type Method = keyof LifecycleProfile | 'changeDetection' | string;
 // Performance track global flag.
 let chromeDevToolsPerformanceTrackEnabled = false;
 
-/** Enable Angular's performance track in the Chrome DevTools profiler. */
-export const enablePerformanceTrack = () => (chromeDevToolsPerformanceTrackEnabled = true);
-
 /** Disable Angular's performance track in the Chrome DevTools profiler. */
 export const disablePerformanceTrack = () => (chromeDevToolsPerformanceTrackEnabled = false);
 
@@ -64,7 +61,7 @@ const endMark = (nodeName: string, method: Method) => {
   }
 };
 
-getProfiler().subscribe({
+const timingHooks = {
   onChangeDetectionStart(component: ComponentInstance): void {
     if (!performanceTrackEnabled()) {
       return;
@@ -101,4 +98,21 @@ getProfiler().subscribe({
     }
     endMark(getDirectiveName(component), output);
   },
-});
+};
+
+let performanceTrackInitialized = false;
+
+function initializePerformanceTrack(): void {
+  if (performanceTrackInitialized) {
+    return;
+  }
+
+  getProfiler().subscribe(timingHooks);
+  performanceTrackInitialized = true;
+}
+
+/** Enable Angular's performance track in the Chrome DevTools profiler. */
+export function enablePerformanceTrack(): void {
+  initializePerformanceTrack();
+  chromeDevToolsPerformanceTrackEnabled = true;
+}